Fig 1: Establishment of mouse Epfn-iPSCs. (A) Generation of doxycycline-inducible Epfn-expressing piggyBac vector (PB-Epfn). (B–D) Inducible Epfn expression in Epfn-iPSCs after 24 h of culture with different doses of Dox (0–2 µg/ml) was examined by real-time PCR (B) and Western blot (C) along with mCherry expression (D). (E,F) Epfn-iPSCs (E-iPSCs) showed comparable pluripotency to naive iPSCs according to the expression of pluripotent marker genes (E), ALP staining, and immunofluorescence results for SSEA-1 and Nanog (F). Scale bar: 100 µm.